Weston B. Struwe Physical and Theoretical Chemistry Laboratory, Department of Chemistry University of Oxford Biography Publications Institution JoVE Articles Weston B. Struwe has not added a biography. If you are Weston B. Struwe and would like to personalize this page please email our Author Liaison for assistance. Publications Measuring Protein-Protein Interactions and Quantifying Their Dissociation Constants with Mass Photometry Current Protocols. Jan, 2024 | Pubmed ID: 38224147 Uncovering the Role of -Glycan Occupancy on the Cooperative Assembly of Spike and Angiotensin Converting Enzyme 2 Complexes: Insights from Glycoengineering and Native Mass Spectrometry Journal of the American Chemical Society. Apr, 2023 | Pubmed ID: 37000485 Engineer RNA-Protein Nanowires As Light-Responsive Biomaterials Small (Weinheim an Der Bergstrasse, Germany). Mar, 2023 | Pubmed ID: 36642821 Mass Photometry Reveals SARS-CoV-2 Spike Stabilisation to Impede ACE2 Binding Through Altered Conformational Dynamics Chemical Communications (Cambridge, England). Nov, 2022 | Pubmed ID: 36317551 An N-glycan on the C2 Domain of JAGGED1 is Important for Notch Activation Science Signaling. Oct, 2022 | Pubmed ID: 36219682 Direct Observation of the Molecular Mechanism Underlying Protein Polymerization Science Advances. Sep, 2022 | Pubmed ID: 36044567 State-of-the-art Glycosaminoglycan Characterization Mass Spectrometry Reviews. Nov, 2022 | Pubmed ID: 34608657 Formation and Fragmentation of Doubly and Triply Charged Ions in the Negative Ion Spectra of Neutral N-glycans from Viral and Other Glycoproteins Analytical and Bioanalytical Chemistry. Dec, 2021 | Pubmed ID: 34342671 Label-free Methods for Optical Characterization of Protein-protein Interactions Physical Chemistry Chemical Physics : PCCP. Aug, 2021 | Pubmed ID: 34342317 Identification of N-glycans with GalNAc-containing Antennae from Recombinant HIV Trimers by Ion Mobility and Negative Ion Fragmentation Analytical and Bioanalytical Chemistry. Dec, 2021 | Pubmed ID: 34327564 Native Mass Spectrometry Meets Glycomics: Resolving Structural Detail and Occupancy of Glycans on Intact Glycoproteins Analytical Chemistry. Aug, 2021 | Pubmed ID: 34279906 Assessing Antigen Structural Integrity Through Glycosylation Analysis of the SARS-CoV-2 Viral Spike ACS Central Science. Apr, 2021 | Pubmed ID: 34056088 Mass Photometry of Membrane Proteins Chem. Jan, 2021 | Pubmed ID: 33511302 Hyper-truncated Asn355- and Asn391-glycans Modulate the Activity of Neutrophil Granule Myeloperoxidase The Journal of Biological Chemistry. 2021 | Pubmed ID: 33273015 Correlating Glycoforms of DC-SIGN with Stability Using a Combination of Enzymatic Digestion and Ion Mobility Mass Spectrometry Angewandte Chemie (International Ed. in English). Sep, 2020 | Pubmed ID: 33462887 Single Molecule Mass Photometry of Nucleic Acids Nucleic Acids Research. Sep, 2020 | Pubmed ID: 32756898 Quantifying the Heterogeneity of Macromolecular Machines by Mass Photometry Nature Communications. Apr, 2020 | Pubmed ID: 32286308 Shotgun Ion Mobility Mass Spectrometry Sequencing of Heparan Sulfate Saccharides Nature Communications. Mar, 2020 | Pubmed ID: 32198425 Quantifying Protein-Protein Interactions by Molecular Counting with Mass Photometry Angewandte Chemie (International Ed. in English). Jun, 2020 | Pubmed ID: 32167227 A Bipartite Structural Organization Defines the SERINC Family of HIV-1 Restriction Factors Nature Structural & Molecular Biology. Jan, 2020 | Pubmed ID: 31907454 Ion Mobility-Mass Spectrometry of Glycoconjugates Methods in Molecular Biology (Clifton, N.J.). 2020 | Pubmed ID: 31729663 Separation of Isomeric Glycans by Ion Mobility Spectrometry - the Impact of Fluorescent Labelling The Analyst. Sep, 2019 | Pubmed ID: 31380551 Towards a Standardized Bioinformatics Infrastructure for N- and O-glycomics Nature Communications. 07, 2019 | Pubmed ID: 31332201 Relating Glycoprotein Structural Heterogeneity to Function - Insights from Native Mass Spectrometry Current Opinion in Structural Biology. Oct, 2019 | Pubmed ID: 31326232 Separation of Isomeric Glycans by Ion Mobility and Liquid Chromatography-Mass Spectrometry Analytical Chemistry. Aug, 2019 | Pubmed ID: 31298840 Probing -glycoprotein Microheterogeneity by Lectin Affinity Purification-mass Spectrometry Analysis Chemical Science. May, 2019 | Pubmed ID: 31183067 In-depth Structural Analysis of Glycans in the Gas Phase Chemical Science. Feb, 2019 | Pubmed ID: 30809341 The Minimum Information Required for a Glycomics Experiment (MIRAGE) Project: LC Guidelines Glycobiology. May, 2019 | Pubmed ID: 30778580 A Mass-Spectrometry-Based Modelling Workflow for Accurate Prediction of IgG Antibody Conformations in the Gas Phase Angewandte Chemie (International Ed. in English). Dec, 2018 | Pubmed ID: 30408305 Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen Cell Reports. Aug, 2018 | Pubmed ID: 30134158 N-glycan Microheterogeneity Regulates Interactions of Plasma Proteins Proceedings of the National Academy of Sciences of the United States of America. Aug, 2018 | Pubmed ID: 30111543 Publisher Correction: The Jumonji-C Oxygenase JMJD7 Catalyzes (3S)-lysyl Hydroxylation of TRAFAC GTPases Nature Chemical Biology. Oct, 2018 | Pubmed ID: 29950663 The Jumonji-C Oxygenase JMJD7 Catalyzes (3S)-lysyl Hydroxylation of TRAFAC GTPases Nature Chemical Biology. Jul, 2018 | Pubmed ID: 29915238 Structural Insights into the Broad-Spectrum Antiviral Target Endoplasmic Reticulum Alpha-Glucosidase II Advances in Experimental Medicine and Biology. 2018 | Pubmed ID: 29845539 Structural Studies of Fucosylated N-Glycans by Ion Mobility Mass Spectrometry and Collision-Induced Fragmentation of Negative Ions Journal of the American Society for Mass Spectrometry. Jun, 2018 | Pubmed ID: 29790113 Signature of Antibody Domain Exchange by Native Mass Spectrometry and Collision-Induced Unfolding Analytical Chemistry. Jun, 2018 | Pubmed ID: 29757629 Quantitative Mass Imaging of Single Biological Macromolecules Science (New York, N.Y.). Apr, 2018 | Pubmed ID: 29700264 Fucose Migration in Intact Protonated Glycan Ions: A Universal Phenomenon in Mass Spectrometry Angewandte Chemie (International Ed. in English). Jun, 2018 | Pubmed ID: 29688603 Collision Cross Sections and Ion Mobility Separation of Fragment Ions from Complex N-Glycans Journal of the American Society for Mass Spectrometry. Jun, 2018 | Pubmed ID: 29675741 Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments Journal of the American Society for Mass Spectrometry. May, 2018 | Pubmed ID: 29508223 Structural Principles That Enable Oligomeric Small Heat-shock Protein Paralogs to Evolve Distinct Functions Science (New York, N.Y.). Feb, 2018 | Pubmed ID: 29472485 Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages Journal of Proteome Research. Mar, 2018 | Pubmed ID: 29420040 Glycosylation Profiling to Evaluate Glycoprotein Immunogens Against HIV-1 Expert Review of Proteomics. Oct, 2017 | Pubmed ID: 28870097 Convergent Immunological Solutions to Argentine Hemorrhagic Fever Virus Neutralization Proceedings of the National Academy of Sciences of the United States of America. Jul, 2017 | Pubmed ID: 28630325 Reducing V3 Antigenicity and Immunogenicity on Soluble, Native-Like HIV-1 Env SOSIP Trimers Journal of Virology. Aug, 2017 | Pubmed ID: 28539451 Glycan Fingerprinting Via Cold-Ion Infrared Spectroscopy Angewandte Chemie (International Ed. in English). Sep, 2017 | Pubmed ID: 28513924 The Tetrameric Plant Lectin BanLec Neutralizes HIV Through Bidentate Binding to Specific Viral Glycans Structure (London, England : 1993). May, 2017 | Pubmed ID: 28434916 Identification of Lewis and Blood Group Carbohydrate Epitopes by Ion Mobility-Tandem-Mass Spectrometry Fingerprinting Analytical Chemistry. Feb, 2017 | Pubmed ID: 28192913 The Role of Interfacial Lipids in Stabilizing Membrane Protein Oligomers Nature. Jan, 2017 | Pubmed ID: 28077870 The Minimum Information Required for a Glycomics Experiment (MIRAGE) Project: Improving the Standards for Reporting Glycan Microarray-based Data Glycobiology. 04, 2017 | Pubmed ID: 27993942 Global N-Glycan Site Occupancy of HIV-1 Gp120 by Metabolic Engineering and High-Resolution Intact Mass Spectrometry ACS Chemical Biology. Feb, 2017 | Pubmed ID: 27984693 Molecular Architecture of the Cleavage-Dependent Mannose Patch on a Soluble HIV-1 Envelope Glycoprotein Trimer Journal of Virology. Jan, 2017 | Pubmed ID: 27807235 Ion Mobility Separation of Deprotonated Oligosaccharide Isomers - Evidence for Gas-phase Charge Migration Chemical Communications (Cambridge, England). Oct, 2016 | Pubmed ID: 27711324 The Minimum Information Required for a Glycomics Experiment (MIRAGE) Project: Sample Preparation Guidelines for Reliable Reporting of Glycomics Datasets Glycobiology. 09, 2016 | Pubmed ID: 27654115 Antibody Production Using a Ciliate Generates Unusual Antibody Glycoforms Displaying Enhanced Cell-killing Activity MAbs. 2016 | Pubmed ID: 27594301 Optimal Synthetic Glycosylation of a Therapeutic Antibody Angewandte Chemie (Weinheim an Der Bergstrasse, Germany). Feb, 2016 | Pubmed ID: 27546920 Travelling-wave Ion Mobility Mass Spectrometry and Negative Ion Fragmentation of Hybrid and Complex N-glycans Journal of Mass Spectrometry : JMS. Nov, 2016 | Pubmed ID: 27477117 Structures of Mammalian ER α-glucosidase II Capture the Binding Modes of Broad-spectrum Iminosugar Antivirals Proceedings of the National Academy of Sciences of the United States of America. Aug, 2016 | Pubmed ID: 27462106 Immune Recruitment or Suppression by Glycan Engineering of Endogenous and Therapeutic Antibodies Biochimica Et Biophysica Acta. Aug, 2016 | Pubmed ID: 27105835 Composition and Antigenic Effects of Individual Glycan Sites of a Trimeric HIV-1 Envelope Glycoprotein Cell Reports. Mar, 2016 | Pubmed ID: 26972002 Travelling-wave Ion Mobility and Negative Ion Fragmentation of High-mannose N-glycans Journal of Mass Spectrometry : JMS. Mar, 2016 | Pubmed ID: 26956389 High-resolution Mass Spectrometry of Small Molecules Bound to Membrane Proteins Nature Methods. Apr, 2016 | Pubmed ID: 26901650 Optimal Synthetic Glycosylation of a Therapeutic Antibody Angewandte Chemie (International Ed. in English). Feb, 2016 | Pubmed ID: 26756880 Structural Characterization and Biological Implications of Sulfated N-glycans in a Serine Protease from the Neotropical Moth Hylesia Metabus (Cramer [1775]) (Lepidoptera: Saturniidae) Glycobiology. Mar, 2016 | Pubmed ID: 26537504 GlycoMob: an Ion Mobility-mass Spectrometry Collision Cross Section Database for Glycomics Glycoconjugate Journal. Jun, 2016 | Pubmed ID: 26314736 Studying the Active-site Loop Movement of the São Paolo Metallo-β-lactamase-1†Electronic Supplementary Information (ESI) Available: Procedures for Protein Expression and Purification, F-labelling, Crystallisation, Data Collection, and Structure Determination, Table of Crystallographic Data, Table of Crystallographic Parameters and Refinement Statistics, Figures Showing Binding Mode and Distances, Procedures for Mass Spectrometry Measurements, Differential Scanning Fluorimetry Measurements, Stopped-flow Measurements and Other Kinetics Measurements. See DOI: 10.1039/c4sc01752hClick Here for Additional Data File Chemical Science. Feb, 2015 | Pubmed ID: 25717359 Native Mass Spectrometry: Towards High-throughput Structural Proteomics Methods in Molecular Biology (Clifton, N.J.). 2015 | Pubmed ID: 25502208 Ejection of Structural Zinc Leads to Inhibition of γ-butyrobetaine Hydroxylase Bioorganic & Medicinal Chemistry Letters. Nov, 2014 | Pubmed ID: 25266780 MIRAGE: the Minimum Information Required for a Glycomics Experiment Glycobiology. May, 2014 | Pubmed ID: 24653214 Increase in Sialylation and Branching in the Mouse Serum N-glycome Correlates with Inflammation and Ovarian Tumour Progression PloS One. 2013 | Pubmed ID: 24023608 The Minimum Information Required for a Glycomics Experiment (MIRAGE) Project: Improving the Standards for Reporting Mass-spectrometry-based Glycoanalytic Data Molecular & Cellular Proteomics : MCP. Apr, 2013 | Pubmed ID: 23378518 Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry Myndert Claasen1, Zornitsa Kofinova1, Matteo Contino2, Weston B. Struwe3,4 1Refeyn Ltd., 2Adaptix Ltd., 3Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, 4The Kavli Institute for Nanoscience Discovery, University of Oxford JoVE 65772 Biochemistry
Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry Myndert Claasen1, Zornitsa Kofinova1, Matteo Contino2, Weston B. Struwe3,4 1Refeyn Ltd., 2Adaptix Ltd., 3Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, 4The Kavli Institute for Nanoscience Discovery, University of Oxford JoVE 65772 Biochemistry