Chinese Academy of Forestry 2 articles published in JoVE Biology Development of an Individual-Tree Basal Area Increment Model using a Linear Mixed-Effects Approach Wenwen Wang1, Yanfeng Bai2, Chunqian Jiang2, Jinghui Meng1 1Research Center of Forest Management Engineering of National Forestry and Grassland Administration, Beijing Forestry University, 2Research Institute of Forestry, Chinese Academy of Forestry Mixed-effects models are flexible and useful tools for analyzing data with a hierarchical stochastic structure in forestry and could also be used to significantly improve the performance of forest growth models. Here, a protocol is presented that synthesizes information relating to linear mixed-effects models. Biology Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus Najie Zhu*1, Liqun Bai*1, Stefan Schütz2, Baojun Liu3, Zhenyu Liu3, Xingyao Zhang4, Hongshi Yu1, Jiafu Hu1,5 1Department of Forest Protection, Zhejiang Agricultural & Forestry University, 2Institute of Forest Zoology and Forest Conservation, Georg-August University Göttingen, 3College of Plant Protection, Shandong Agricultural University, 4Institute of Forest Protection, Chinese Academy of Forestry, 5Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University A protocol for investigating the mating behavior of the pinewood nematode, Bursaphelenchus xylophilus, is presented. Behavioral features of B. xylophilus are described in the mating process.