Wuhan University of Science and Technology 2 articles published in JoVE Neuroscience Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro Xiang Mao*1, Shasha Zhao*2 1Wuhan Center for Disease Control and Prevention, 2College of Life Science and Health, Wuhan University of Science and Technology Here, we established a low cost and easy to operate method that directs fast and efficient differentiation from embryonic stem cells into neurons. This method is suitable for popularization among laboratories and can be a useful tool for neurological research. Engineering Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro Zilan Xiong*1, Shasha Zhao*2, Xu Yan3 1State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, 2College of Life Science and Health, Wuhan University of Science and Technology, 3Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University This protocol aims to provide detailed experimental steps of a cold atmospheric plasma treatment on neural stem cells and immunofluorescence detection for differentiation enhancement.