Nankai University School of Medicine 1 article published in JoVE Medicine Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis Kaiyue Zhang1, Chen Wang1, Ran Wang2, Shang Chen1, Zongjin Li1 1Nankai University School of Medicine, 2State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University This protocol describes the establishment of a tumor-bearing mouse model to monitor tumor progression and angiogenesis in real-time by dual bioluminescence imaging.