Tunghai University View Institution's Website 2 articles published in JoVE Developmental Biology Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish Chih-Wei Chou1, Jamie Lin1, Hsin-Yu Hou1, Yi-Wen Liu1 1Department of Life Science, Tunghai University The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-β-hydroxysteroid dehydrogenase (Δ5-4 isomerase; 3β-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish. Biology Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation Yuan-Hao Hsu1, Jolinda A. Traugh2 1Department of Chemistry, Tunghai University, 2Department of Biochemistry, University of California, Riverside MALDI-TOF mass spectrometry was successfully utilized to monitor the amide hydrogen/deuterium exchange in protein kinase Pak2 activation.