Gottingen Graduate School for Neurosciences, Biophysics, and Molecular Biosciences (GGNB) 1 article published in JoVE Biology Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles Naira Movsisyan1,2, Luis A. Pardo1 1AG Oncophysiology, Max-Planck Institute of Experimental Medicine, 2Göttingen Graduate School for Neurosciences, Biophysics, and Molecular Biosciences (GGNB) Here we present a robust and detailed method of microtubule dynamics analysis in cells synchronized in prometaphase using live-cell spinning disk confocal microscopy and MATLAB-based image processing.