Copenhagen University Hospital (Rigshospitalet) View Institution's Website 7 articles published in JoVE Medicine Dual Test Gas Pulmonary Diffusing Capacity Measurement During Exercise in Humans Using the Single-Breath Method Stine B. Nymand1, Jacob Peter Hartmann1,2,3, Helene Louise Hartmeyer1, Iben E. Rasmussen1, Amalie Bach Andersen1, Milan Mohammad1, Susan Al-Atabi3, Birgitte Hanel3, Ulrik Winning Iepsen1,4, Jann Mortensen3,5, Ronan M. G. Berg1,2,3,6 1Centre for Physical Activity Research, Copenhagen University Hospital - Rigshospitalet, 2Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 3Department of Clinical Physiology and Nuclear Medicine, Copenhagen University Hospital - Rigshospitalet, 4Department of Anesthesiology and Intensive Care, Hvidovre Hospital, 5Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 6Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales This protocol presents a method to assess pulmonary alveolar-capillary reserve measured by combined single-breath measurement of the diffusing capacity to carbon monoxide (DL,CO) and nitric oxide (DL,NO) during exercise. Assumptions and recommendations for using the technique during exercise form the foundation of this article. Neuroscience Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model Jesper Peter Bömers1,2, Sara Ellinor Johansson2, Lars Edvinsson2,4, Tiit Illimar Mathiesen1,3,5, Kristian Agmund Haanes2 1Department of Neurosurgery, Rigshospitalet, 2Department of Clinical Experimental Research, Glostrup Research Institute, Rigshospitalet, 3Department of Clinical Medicine, University of Copenhagen, 4Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, 5Department of Clinical Neuroscience, Karolinska Institutet Subarachnoid hemorrhage continues to carry a high burden of mortality and morbidity in man. To facilitate further research into the condition and its pathophysiology, a pre-chiasmatic, single injection model is presented. Immunology and Infection Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay Maria del Pilar Quintana1, Nsoh Godwin Anabire1,2,3, Lars Hviid1,4 1Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, 3Department of Immunology, Noguchi Memorial Institute for Medical Research, 4Centre for Medical Parasitology, Department of Infectious Diseases, Rigshospitalet The overall goal of this protocol is to provide instruction on how to measure the capacity of antibodies present in sera or plasma of individuals, naturally exposed to Plasmodium falciparum infection, to opsonize and induce phagocytosis of the parasite-infected erythrocytes (IEs). Neuroscience In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette Changsi Cai1, Stefan A. Zambach1, Jonas C. Fordsmann1, Micael Lønstrup1, Kirsten J. Thomsen1, Aske G. K. Jensen1, Martin Lauritzen1,2 1Department of Neuroscience, Faculty of Medicine and Health Science, University of Copenhagen, 2Department of Clinical Neurophysiology, Rigshospitalet We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions. Neuroscience Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets Nane Griem-Krey1, Anders Bue Klein1, Matthias Herth1,2,3, Petrine Wellendorph1 1Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2Neurobiology Research Unit and CIMBI, Copenhagen University Hospital, 3Department of Clinical Physiology, Nuclear Medicine & PET, Copenhagen University Hospital The method of autoradiography is routinely used to study binding of radioligands to tissue sections for determination of qualitative or quantitative pharmacology. Biology Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm Anders Rehfeld1,2, Dorte Louise Egeberg Palme1,2, Kristian Almstrup1,2, Anders Juul1,2, Niels Erik Skakkebaek1,2 1Department of Growth and Reproduction, Copenhagen University Hospital, 2International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), University of Copenhagen Here, two medium-throughput assays for assessment of effects on Ca2+-signaling and acrosome reaction in human sperm are described. These assays can be used to quickly and easily screen large amounts of compounds for effects on Ca2+-signaling and acrosome reaction in human sperm. Biology Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH) Elena Ronander1,2, Dominique C. Bengtsson1,2, Louise Joergensen1,2, Anja T. R. Jensen1,2, David E. Arnot1,2,3 1Centre for Medical Parasitology, Department of International Health, Immunology & Microbiology, Faculty of Health Sciences, University of Copenhagen, 2Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), 3Institute of Infection and Immunology Research, School of Biology, University of Edinburgh Fluorescent in situ hybridization (FISH) to identify mRNA transcripts in individual cells allows analysis of polygenic activity such as the simultaneous transcription of more than one member of the var multigene family in Plasmodium falciparum infected erythrocytes 1. The technique is adaptable and can be used on different types of genes, cells and organisms.
