Cambridge University 1 article published in JoVE Immunology and Infection Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells Emily Read*1,2, Geraldine M. Jowett*1,2,3, Diana Coman1, Joana F. Neves1 1Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University This protocol offers detailed instructions for establishing murine small intestine organoids, isolating type-1 innate lymphoid cells from the murine small intestine lamina propria, and establishing 3-dimensional (3D) co-cultures between both cell types to study bi-directional interactions between intestinal epithelial cells and type-1 innate lymphoid cells.