Jagiellonian University in Krakow 2 articles published in JoVE Immunology and Infection Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization Anna Kula-Pacurar1, Jakub Wadas2, Agnieszka Suder1, Artur Szczepanski1,2, Aleksandra Milewska1,2, Marek Ochman3,4, Tomasz Stacel3,4, Krzysztof Pyrc1 1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases Here, we describe a simple method that combines RNA fluorescence in situ hybridization (RNA-FISH) with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. This protocol may increase understanding of the molecular characteristics of SARS-CoV-2 RNA-host interactions at a single-cell level. Biology Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation Gabriela Gorczyca1, Kamil Wartalski2, Zbigniew Tabarowski3, Malgorzata Duda1 1Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow, 2Department of Histology, Jagiellonian University Medical College, 3Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow Presented here are two protocols for the encapsulation of porcine oocytes in 3D culture conditions. In the first, cumulus-oocyte complexes (COCs) are encapsulated in fibrin-alginate beads. In the second, they are enclosed with fluorinated ethylene propylene powder particles (microbioreactors). Both systems ensure optimal conditions to maintain their 3D organization.