Geisel School of Medicine and Dartmouth-Hitchcock Medical Center 2 articles published in JoVE Immunology and Infection Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells Krista D. DiSano1, Michael R. Linzey1,2, Nora C. Welsh1,2, Joshua S. Meier1, Andrew R. Pachner1, Francesca Gilli1 1Department of Neurology, Geisel School of Medicine, Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine, Dartmouth College This paper presents two optimized protocols for examining resident and peripherally derived immune cells within the central nervous system, including the brain, spinal cord, and meninges. Each of these protocols helps to ascertain the function and composition of the cells occupying these compartments under steady state and inflammatory conditions. Neuroscience Quantitative Measurement of Intrathecally Synthesized Proteins in Mice Francesca Gilli1, Nora C. Welsh1,2, Michael R. Linzey1,2, Darlene B. Royce1, Krista D. DiSano1, Andrew R. Pachner1 1Department of Neurology, Geisel School of Medicine & Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine, Dartmouth College Elevated spinal fluid protein levels can either be the result of diffusion of plasma protein across an altered blood-brain barrier or intrathecal synthesis. An optimized testing protocol is presented in this article that helps to discriminate both cases and provides quantitative measurements of intrathecally synthesized proteins.