Pacific Northwest Diabetes Research Institute 1 article published in JoVE Biology BEST: Barcode Enabled Sequencing of Tetrads Adrian C. Scott1, Catherine L. Ludlow1, Gareth A. Cromie1, Aimée M. Dudley1 1Pacific Northwest Diabetes Research Institute Barcode Enabled Sequencing of Tetrads (BEST) replaces the manual processes of isolating, disrupting and spacing tetrads. BEST isolates tetrads by fluorescence-activated cell sorting onto agar plates, separates the spores by agitation with glass beads, and determines which randomly arrayed colonies were derived from the same original tetrad using molecular barcodes.