Texas Commission on Environmental Quality 1 article published in JoVE Developmental Biology Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts Yujie Huang*1, Yizhou Tao*1, Chang Cai1, Jin Chen1, Cheng Ji1, Stanley Aniagu2, Yan Jiang1, Tao Chen1 1School of Public Health, Soochow University, 2Toxicology, Risk Assessment and Research Division, Texas Commission on Environmental Quality This protocol uses an immunofluorescence assay to detect PM2.5-induced DNA damage in the dissected hearts of zebrafish embryos.