JoVE Journal
Genetics
Genetics
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.