JoVE Journal
Neuroscience
Neuroscience
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
TrackMate Analysis of Calcium Imaging (TACI) is an open-source ImageJ plugin for 3D calcium imaging analysis that examines motion on the z-axis and identifies the maximum value of each z-stack to represent a cell's intensity at the corresponding time point. It can separate neurons overlapping in the lateral (x/y) direction but on different z-planes.