George M. Church Wyss Institute for Biologically Inspired Engineering Harvard University Biography Publications Institution JoVE Articles George M. Church has not added a biography. If you are George M. Church and would like to personalize this page please email our Author Liaison for assistance. Publications Developmental Barcoding of Whole Mouse Via Homing CRISPR Science (New York, N.Y.). 08, 2018 | Pubmed ID: 30093604 Biosensor Libraries Harness Large Classes of Binding Domains for Construction of Allosteric Transcriptional Regulators Nature Communications. Aug, 2018 | Pubmed ID: 30082754 An Enhanced CRISPR Repressor for Targeted Mammalian Gene Regulation Nature Methods. Aug, 2018 | Pubmed ID: 30013045 Directed Differentiation of Human Induced Pluripotent Stem Cells into Mature Kidney Podocytes and Establishment of a Glomerulus Chip Nature Protocols. Jul, 2018 | Pubmed ID: 29995874 Enhanced Bacterial Immunity and Mammalian Genome Editing Via RNA-Polymerase-Mediated Dislodging of Cas9 from Double-Strand DNA Breaks Molecular Cell. Jul, 2018 | Pubmed ID: 29979968 Current CRISPR Gene Drive Systems Are Likely to Be Highly Invasive in Wild Populations ELife. Jun, 2018 | Pubmed ID: 29916367 High-throughput Creation and Functional Profiling of DNA Sequence Variant Libraries Using CRISPR-Cas9 in Yeast Nature Biotechnology. Jul, 2018 | Pubmed ID: 29786095 Codon Usage of Highly Expressed Genes Affects Proteome-wide Translation Efficiency Proceedings of the National Academy of Sciences of the United States of America. 05, 2018 | Pubmed ID: 29735666 High-resolution Mapping of DNA Polymerase Fidelity Using Nucleotide Imbalances and Next-generation Sequencing Nucleic Acids Research. Jul, 2018 | Pubmed ID: 29718339 An Integrated Genome-wide CRISPRa Approach to Functionalize LncRNAs in Drug Resistance Cell. Apr, 2018 | Pubmed ID: 29677511 Enabling Multiplexed Testing of Pooled Donor Cells Through Whole-genome Sequencing Genome Medicine. 04, 2018 | Pubmed ID: 29673390 Precise Cas9 Targeting Enables Genomic Mutation Prevention Proceedings of the National Academy of Sciences of the United States of America. 04, 2018 | Pubmed ID: 29555762 Adaptive Evolution of Genomically Recoded Proceedings of the National Academy of Sciences of the United States of America. 03, 2018 | Pubmed ID: 29440500 Spontaneous CRISPR Loci Generation in Vivo by Non-canonical Spacer Integration Nature Microbiology. Mar, 2018 | Pubmed ID: 29379209 From Designing the Molecules of Life to Designing Life: Future Applications Derived from Advances in DNA Technologies Angewandte Chemie (International Ed. in English). Apr, 2018 | Pubmed ID: 29316123 Engineering Posttranslational Proofreading to Discriminate Nonstandard Amino Acids Proceedings of the National Academy of Sciences of the United States of America. 01, 2018 | Pubmed ID: 29301968 Heterologous Erythromycin Production Across Strain and Plasmid Construction Biotechnology Progress. Jan, 2018 | Pubmed ID: 28960932 A CRISPR-Cas9-based Gene Drive Platform for Genetic Interaction Analysis in Candida Albicans Nature Microbiology. Oct, 2017 | Pubmed ID: 29062088 Mature Induced-pluripotent-stem-cell-derived Human Podocytes Reconstitute Kidney Glomerular-capillary-wall Function on a Chip Nature Biomedical Engineering. 2017 | Pubmed ID: 29038743 DNA Sequencing at 40: Past, Present and Future Nature. 10, 2017 | Pubmed ID: 29019985 Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues Nano Letters. 10, 2017 | Pubmed ID: 28933153 Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy Methods in Molecular Biology (Clifton, N.J.). 2017 | Pubmed ID: 28828661 Extracellular Vesicle Isolation and Analysis by Western Blotting Methods in Molecular Biology (Clifton, N.J.). 2017 | Pubmed ID: 28828654 Inactivation of Porcine Endogenous Retrovirus in Pigs Using CRISPR-Cas9 Science (New York, N.Y.). 09, 2017 | Pubmed ID: 28798043 Rates, Distribution and Implications of Postzygotic Mosaic Mutations in Autism Spectrum Disorder Nature Neuroscience. 09, 2017 | Pubmed ID: 28714951 CRISPR-Cas Encoding of a Digital Movie into the Genomes of a Population of Living Bacteria Nature. 07, 2017 | Pubmed ID: 28700573 Scalable Device for Automated Microbial Electroporation in a Digital Microfluidic Platform ACS Synthetic Biology. 09, 2017 | Pubmed ID: 28569062 Millstone: Software for Multiplex Microbial Genome Analysis and Engineering Genome Biology. 05, 2017 | Pubmed ID: 28545559 Optimizing Complex Phenotypes Through Model-guided Multiplex Genome Engineering Genome Biology. 05, 2017 | Pubmed ID: 28545477 Nucleotide-time Alignment for Molecular Recorders PLoS Computational Biology. May, 2017 | Pubmed ID: 28459860 Evolutionary Dynamics of CRISPR Gene Drives Science Advances. Apr, 2017 | Pubmed ID: 28435878 An Unbiased Index to Quantify Participant's Phenotypic Contribution to an Open-access Cohort Scientific Reports. 04, 2017 | Pubmed ID: 28387241 Programmable Transcriptional Repression in Mycobacteria Using an Orthogonal CRISPR Interference Platform Nature Microbiology. Feb, 2017 | Pubmed ID: 28165460 SgRNA Scorer 2.0: A Species-Independent Model To Predict CRISPR/Cas9 Activity ACS Synthetic Biology. 05, 2017 | Pubmed ID: 28146356 Cushing's Syndrome Mutant PKA Exhibits Altered Substrate Specificity FEBS Letters. 02, 2017 | Pubmed ID: 28100013 Efficient, Footprint-free Human IPSC Genome Editing by Consolidation of Cas9/CRISPR and PiggyBac Technologies Nature Protocols. Jan, 2017 | Pubmed ID: 27929521 Rapidly Evolving Homing CRISPR Barcodes Nature Methods. 02, 2017 | Pubmed ID: 27918539 Complete Mitochondrial Genomes of Living and Extinct Pigeons Revise the Timing of the Columbiform Radiation BMC Evolutionary Biology. 10, 2016 | Pubmed ID: 27782796 Design and Characterization of a Nanopore-coupled Polymerase for Single-molecule DNA Sequencing by Synthesis on an Electrode Array Proceedings of the National Academy of Sciences of the United States of America. 11, 2016 | Pubmed ID: 27729524 Emergent Rules for Codon Choice Elucidated by Editing Rare Arginine Codons in Escherichia Coli Proceedings of the National Academy of Sciences of the United States of America. 09, 2016 | Pubmed ID: 27601680 A Multifunctional AAV-CRISPR-Cas9 and Its Host Response Nature Methods. 10, 2016 | Pubmed ID: 27595405 CRISPR-Cas9 System: Opportunities and Concerns Clinical Chemistry. 10, 2016 | Pubmed ID: 27551006 Design, Synthesis, and Testing Toward a 57-codon Genome Science (New York, N.Y.). Aug, 2016 | Pubmed ID: 27540174 Molecular Recordings by Directed CRISPR Spacer Acquisition Science (New York, N.Y.). Jul, 2016 | Pubmed ID: 27284167 Real-time Single-molecule Electronic DNA Sequencing by Synthesis Using Polymer-tagged Nucleotides on a Nanopore Array Proceedings of the National Academy of Sciences of the United States of America. May, 2016 | Pubmed ID: 27091962 Nucleic Acid Memory Nature Materials. Apr, 2016 | Pubmed ID: 27005909 Biosensor-based Engineering of Biosynthetic Pathways Current Opinion in Biotechnology. 12, 2016 | Pubmed ID: 26998575 Multiplexed Engineering in Biology Trends in Biotechnology. Mar, 2016 | Pubmed ID: 26897356 Genetically Encoded Sensors Enable Real-time Observation of Metabolite Production Proceedings of the National Academy of Sciences of the United States of America. Mar, 2016 | Pubmed ID: 26858408 In Vivo Gene Editing in Dystrophic Mouse Muscle and Muscle Stem Cells Science (New York, N.Y.). Jan, 2016 | Pubmed ID: 26721686 Tools for the Microbiome: Nano and Beyond ACS Nano. Jan, 2016 | Pubmed ID: 26695070 Engineering an Allosteric Transcription Factor to Respond to New Ligands Nature Methods. Feb, 2016 | Pubmed ID: 26689263 Precision Chemistry for Precision Medicine ACS Central Science. Mar, 2015 | Pubmed ID: 27162939 A General Strategy to Construct Small Molecule Biosensors in Eukaryotes ELife. Dec, 2015 | Pubmed ID: 26714111 Bioart Trends in Biotechnology. Dec, 2015 | Pubmed ID: 26617334 Unraveling CRISPR-Cas9 Genome Engineering Parameters Via a Library-on-library Approach Nature Methods. Sep, 2015 | Pubmed ID: 26167643 Synthetic Biosensors for Precise Gene Control and Real-time Monitoring of Metabolites Nucleic Acids Research. Sep, 2015 | Pubmed ID: 26152303 Highly Efficient Cas9-mediated Transcriptional Programming Nature Methods. Apr, 2015 | Pubmed ID: 25730490 Biocontainment of Genetically Modified Organisms by Synthetic Protein Design Nature. Feb, 2015 | Pubmed ID: 25607366 Multi-kilobase Homozygous Targeted Gene Replacement in Human Induced Pluripotent Stem Cells Nucleic Acids Research. Feb, 2015 | Pubmed ID: 25414332 Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-derived Oligonucleotides ACS Synthetic Biology. Jan, 2015 | Pubmed ID: 24856730 Spatial Information in Large-scale Neural Recordings Frontiers in Computational Neuroscience. 2014 | Pubmed ID: 25653613 Evolution-guided Optimization of Biosynthetic Pathways Proceedings of the National Academy of Sciences of the United States of America. Dec, 2014 | Pubmed ID: 25453111 Rapid Neurogenesis Through Transcriptional Activation in Human Stem Cells Molecular Systems Biology. Nov, 2014 | Pubmed ID: 25403753 Engineering Allostery Trends in Genetics : TIG. Dec, 2014 | Pubmed ID: 25306102 Multiplex Single-molecule Interaction Profiling of DNA-barcoded Proteins Nature. Nov, 2014 | Pubmed ID: 25252978 Genome Editing Assessment Using CRISPR Genome Analyzer (CRISPR-GA) Bioinformatics (Oxford, England). Oct, 2014 | Pubmed ID: 24990609 CHOPCHOP: a CRISPR/Cas9 and TALEN Web Tool for Genome Editing Nucleic Acids Research. Jul, 2014 | Pubmed ID: 24861617 Modeling the Mitochondrial Cardiomyopathy of Barth Syndrome with Induced Pluripotent Stem Cell and Heart-on-chip Technologies Nature Medicine. Jun, 2014 | Pubmed ID: 24813252 Syntrophic Exchange in Synthetic Microbial Communities Proceedings of the National Academy of Sciences of the United States of America. May, 2014 | Pubmed ID: 24778240 High-resolution Antibody Dynamics of Vaccine-induced Immune Responses Proceedings of the National Academy of Sciences of the United States of America. Apr, 2014 | Pubmed ID: 24639495 Realizing the Potential of Synthetic Biology Nature Reviews. Molecular Cell Biology. 04, 2014 | Pubmed ID: 24622617 Highly Multiplexed Subcellular RNA Sequencing in Situ Science (New York, N.Y.). Mar, 2014 | Pubmed ID: 24578530 Rational Optimization of TolC As a Powerful Dual Selectable Marker for Genome Engineering Nucleic Acids Research. Apr, 2014 | Pubmed ID: 24452804 The Role of Replicates for Error Mitigation in Next-generation Sequencing Nature Reviews. Genetics. 01, 2014 | Pubmed ID: 24322726 Natural Reassignment of CUU and CUA Sense Codons to Alanine in Ashbya Mitochondria Nucleic Acids Research. Jan, 2014 | Pubmed ID: 24049072 The Effects of Somatic Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies PLoS Pathogens. 2013 | Pubmed ID: 24278016 Physical Principles for Scalable Neural Recording Frontiers in Computational Neuroscience. 2013 | Pubmed ID: 24187539 Genomically Recoded Organisms Expand Biological Functions Science (New York, N.Y.). Oct, 2013 | Pubmed ID: 24136966 Cas9 As a Versatile Tool for Engineering Biology Nature Methods. Oct, 2013 | Pubmed ID: 24076990 Heritable Custom Genomic Modifications in Caenorhabditis Elegans Via a CRISPR-Cas9 System Genetics. Nov, 2013 | Pubmed ID: 23979579 Multiplex and Homologous Recombination-mediated Genome Editing in Arabidopsis and Nicotiana Benthamiana Using Guide RNA and Cas9 Nature Biotechnology. Aug, 2013 | Pubmed ID: 23929339 CAS9 Transcriptional Activators for Target Specificity Screening and Paired Nickases for Cooperative Genome Engineering Nature Biotechnology. Sep, 2013 | Pubmed ID: 23907171 Optical Control of Mammalian Endogenous Transcription and Epigenetic States Nature. Aug, 2013 | Pubmed ID: 23877069 Heritable Genome Editing in C. Elegans Via a CRISPR-Cas9 System Nature Methods. Aug, 2013 | Pubmed ID: 23817069 Nanotools for Neuroscience and Brain Activity Mapping ACS Nano. Mar, 2013 | Pubmed ID: 23514423 Barcoding Cells Using Cell-surface Programmable DNA-binding Domains Nature Methods. May, 2013 | Pubmed ID: 23503053 PhIP-Seq Characterization of Autoantibodies from Patients with Multiple Sclerosis, Type 1 Diabetes and Rheumatoid Arthritis Journal of Autoimmunity. Jun, 2013 | Pubmed ID: 23497938 RNA-guided Human Genome Engineering Via Cas9 Science (New York, N.Y.). Feb, 2013 | Pubmed ID: 23287722 Submicrometre Geometrically Encoded Fluorescent Barcodes Self-assembled from DNA Nature Chemistry. Oct, 2012 | Pubmed ID: 23000997 Improving Lambda Red Genome Engineering in Escherichia Coli Via Rational Removal of Endogenous Nucleases PloS One. 2012 | Pubmed ID: 22957093 How Accurate Can Genetic Predictions Be? BMC Genomics. Jul, 2012 | Pubmed ID: 22827772 Multiplexed in Vivo His-tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis ACS Synthetic Biology. Feb, 2012 | Pubmed ID: 22737598 A BioBrick Compatible Strategy for Genetic Modification of Plants Journal of Biological Engineering. 2012 | Pubmed ID: 22716313 Enhanced Multiplex Genome Engineering Through Co-operative Oligonucleotide Co-selection Nucleic Acids Research. Sep, 2012 | Pubmed ID: 22638574 Genome-scale Promoter Engineering by Coselection MAGE Nature Methods. Jun, 2012 | Pubmed ID: 22484848 High-throughput Multiplex Sequencing of MiRNA Current Protocols in Human Genetics. Apr, 2012 | Pubmed ID: 22470142 Precise Manipulation of Chromosomes in Vivo Enables Genome-wide Codon Replacement Science (New York, N.Y.). Jul, 2011 | Pubmed ID: 21764749 Autoantigen Discovery with a Synthetic Human Peptidome Nature Biotechnology. May, 2011 | Pubmed ID: 21602805 A New Dawn for Industrial Photosynthesis Photosynthesis Research. Mar, 2011 | Pubmed ID: 21318462 Efficient Construction of Sequence-specific TAL Effectors for Modulating Mammalian Transcription Nature Biotechnology. Feb, 2011 | Pubmed ID: 21248753 Research, Collaboration, and Open Science Using Web 2.0 Journal of Microbiology & Biology Education. 2010 | Pubmed ID: 23653712 Reprogramming of T Cells from Human Peripheral Blood Cell Stem Cell. Jul, 2010 | Pubmed ID: 20621044 Programming Cells by Multiplex Genome Engineering and Accelerated Evolution Nature. Aug, 2009 | Pubmed ID: 19633652 Rapid Prototyping of 3D DNA-origami Shapes with CaDNAno Nucleic Acids Research. Aug, 2009 | Pubmed ID: 19531737 Free Factories: Unified Infrastructure for Data Intensive Web Services Proceedings of the USENIX ... Annual Technical Conference. USENIX Technical Conference. May, 2008 | Pubmed ID: 20514356 The Most Conserved Genome Segments for Life Detection on Earth and Other Planets Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life. Dec, 2008 | Pubmed ID: 18853276 The Transition Between Transcriptional Initiation and Elongation in E. Coli is Highly Variable and Often Rate Limiting Molecular Cell. Dec, 2006 | Pubmed ID: 17157257 Genomic Analysis of LexA Binding Reveals the Permissive Nature of the Escherichia Coli Genome and Identifies Unconventional Target Sites Genes & Development. Nov, 2005 | Pubmed ID: 16264194 The Complete Genome and Proteome of Mycoplasma Mobile Genome Research. Aug, 2004 | Pubmed ID: 15289470 A Motif Co-occurrence Approach for Genome-wide Prediction of Transcription-factor-binding Sites in Escherichia Coli Genome Research. Feb, 2004 | Pubmed ID: 14762058 Nucleotides of Transcription Factor Binding Sites Exert Interdependent Effects on the Binding Affinities of Transcription Factors Nucleic Acids Research. Mar, 2002 | Pubmed ID: 11861919 Exploring the DNA-binding Specificities of Zinc Fingers with DNA Microarrays Proceedings of the National Academy of Sciences of the United States of America. Jun, 2001 | Pubmed ID: 11404456 CRISPR Guide RNA Cloning for Mammalian Systems Sathiji Nageshwaran*1,2, Alejandro Chavez*1,2,3, Nan Cher Yeo1,2, Xiaoge Guo1,2, Alissa Lance-Byrne1, Angela Tung1, James J. Collins1,4,5,6,7, George M. Church1,2 1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute JoVE 57998 Genetics
CRISPR Guide RNA Cloning for Mammalian Systems Sathiji Nageshwaran*1,2, Alejandro Chavez*1,2,3, Nan Cher Yeo1,2, Xiaoge Guo1,2, Alissa Lance-Byrne1, Angela Tung1, James J. Collins1,4,5,6,7, George M. Church1,2 1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute JoVE 57998 Genetics