Keiji Numata Enzyme Research Team RIKEN Center for Sustainable Resource Science Biography Publications Institution JoVE Articles Keiji Numata has not added a biography. If you are Keiji Numata and would like to personalize this page please email our Author Liaison for assistance. Publications Characterization of the Depolymerizing Activity of Commercial Lipases and Detection of Lipase-like Activities in Animal Organ Extracts Using Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Thin Film AMB Express. Dec, 2016 | Pubmed ID: 27730572 A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater Frontiers in Microbiology. 2016 | Pubmed ID: 27708640 Self-Assembled Peptide-Based System for Mitochondrial-Targeted Gene Delivery: Functional and Structural Insights Biomacromolecules. Nov, 2016 | Pubmed ID: 27696822 Enzyme-Mimic Peptide Assembly To Achieve Amidolytic Activity Biomacromolecules. Oct, 2016 | Pubmed ID: 27642764 Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria PloS One. 2016 | Pubmed ID: 27513570 Relationships Between Physical Properties and Sequence in Silkworm Silks Scientific Reports. Jun, 2016 | Pubmed ID: 27279149 Intracellular Delivery of Proteins Via Fusion Peptides in Intact Plants PloS One. 2016 | Pubmed ID: 27100681 Oil-free Hyaluronic Acid Matrix for Serial Femtosecond Crystallography Scientific Reports. Apr, 2016 | Pubmed ID: 27087008 Biosynthesis of Polyhydroxyalkanoates Containing Hydroxyl Group from Glycolate in Escherichia Coli AMB Express. Dec, 2016 | Pubmed ID: 27075993 Papain-Catalyzed Chemoenzymatic Synthesis of Telechelic Polypeptides Using Bis(Leucine Ethyl Ester) Initiator Macromolecular Bioscience. Jul, 2016 | Pubmed ID: 26947148 Influence of Water Content on the β-Sheet Formation, Thermal Stability, Water Removal, and Mechanical Properties of Silk Materials Biomacromolecules. Mar, 2016 | Pubmed ID: 26835719 The Benzyl Ester Group of Amino Acid Monomers Enhances Substrate Affinity and Broadens the Substrate Specificity of the Enzyme Catalyst in Chemoenzymatic Copolymerization Biomacromolecules. Jan, 2016 | Pubmed ID: 26620763 Chemoenzymatic Synthesis of Oligo(L-cysteine) for Use As a Thermostable Bio-Based Material Macromolecular Bioscience. Jan, 2016 | Pubmed ID: 26388290 Use of Extension-deformation-based Crystallisation of Silk Fibres to Differentiate Their Functions in Nature Soft Matter. Aug, 2015 | Pubmed ID: 26166211 Chemo-Enzymatic Synthesis of Linear and Branched Cationic Peptides: Evaluation As Gene Carriers Macromolecular Bioscience. Jul, 2015 | Pubmed ID: 25828913 Co-expression of Two Polyhydroxyalkanoate Synthase Subunits from Synechocystis Sp. PCC 6803 by Cell-free Synthesis and Their Specific Activity for Polymerization of 3-hydroxybutyryl-coenzyme A Biochemistry. Feb, 2015 | Pubmed ID: 25629766 Gene Introduction into the Mitochondria of Arabidopsis Thaliana Via Peptide-based Carriers Scientific Reports. Jan, 2015 | Pubmed ID: 25583214 Optimization of Poly(N-isopropylacrylamide) As an Artificial Amidase Biomacromolecules. Jan, 2015 | Pubmed ID: 25488109 Recent Advances in Chemoenzymatic Peptide Syntheses Molecules (Basel, Switzerland). Sep, 2014 | Pubmed ID: 25191871 Synthesis of Homopolypeptides by Aminolysis Mediated by Proteases Encapsulated in Silica Nanospheres Macromolecular Bioscience. Nov, 2014 | Pubmed ID: 25154484 Synthesis of Adhesive Peptides Similar to Those Found in Blue Mussel (Mytilus Edulis) Using Papain and Tyrosinase Biomacromolecules. Aug, 2014 | Pubmed ID: 25030620 Local Gene Silencing in Plants Via Synthetic DsRNA and Carrier Peptide Plant Biotechnology Journal. Oct, 2014 | Pubmed ID: 24905384 Silk-pectin Hydrogel with Superior Mechanical Properties, Biodegradability, and Biocompatibility Macromolecular Bioscience. Jun, 2014 | Pubmed ID: 24610718 Phasin Proteins Activate Aeromonas Caviae Polyhydroxyalkanoate (PHA) Synthase but Not Ralstonia Eutropha PHA Synthase Applied and Environmental Microbiology. May, 2014 | Pubmed ID: 24584238 Pathway-level Acceleration of Glycogen Catabolism by a Response Regulator in the Cyanobacterium Synechocystis Species PCC 6803 Plant Physiology. Apr, 2014 | Pubmed ID: 24521880 Short One-pot Chemo-enzymatic Synthesis of L-lysine and L-alanine Diblock Co-oligopeptides Biomacromolecules. Mar, 2014 | Pubmed ID: 24484289 Modification of β-oxidation Pathway in Ralstonia Eutropha for Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Soybean Oil Journal of Bioscience and Bioengineering. Feb, 2014 | Pubmed ID: 23999062 Proteinase K-catalyzed Synthesis of Linear and Star Oligo(L-phenylalanine) Conjugates Biomacromolecules. Oct, 2013 | Pubmed ID: 24000943 Increased Bioplastic Production with an RNA Polymerase Sigma Factor SigE During Nitrogen Starvation in Synechocystis Sp. PCC 6803 DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes. Dec, 2013 | Pubmed ID: 23861321 Characterization of Site-specific Mutations in a Short-chain-length/medium-chain-length Polyhydroxyalkanoate Synthase: in Vivo and in Vitro Studies of Enzymatic Activity and Substrate Specificity Applied and Environmental Microbiology. Jun, 2013 | Pubmed ID: 23584780 Monitoring and Kinetic Analysis of the Molecular Interactions by Which a Repressor Protein, PhaR, Binds to Target DNAs and Poly[(R)-3-hydroxybutyrate] AMB Express. Jan, 2013 | Pubmed ID: 23351303 Biopolymer-based Nanoparticles for Drug/gene Delivery and Tissue Engineering International Journal of Molecular Sciences. Jan, 2013 | Pubmed ID: 23344060 Rapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide Carriers Biomacromolecules. Jan, 2013 | Pubmed ID: 23215041 Active Intermediates of Polyhydroxyalkanoate Synthase from Aeromonas Caviae in Polymerization Reaction Biomacromolecules. Nov, 2012 | Pubmed ID: 23043466 Biocompatible and Biodegradable Dual-drug Release System Based on Silk Hydrogel Containing Silk Nanoparticles Biomacromolecules. May, 2012 | Pubmed ID: 22471816 Chemoenzymatic Synthesis of Poly(L-alanine) in Aqueous Environment Biomacromolecules. Apr, 2012 | Pubmed ID: 22380731 Biosynthesis of Polyhydroxyalkanaotes by a Novel Facultatively Anaerobic Vibrio Sp. Under Marine Conditions Marine Biotechnology (New York, N.Y.). Jun, 2012 | Pubmed ID: 22068389 Silk-based Nanocomplexes with Tumor-homing Peptides for Tumor-specific Gene Delivery Macromolecular Bioscience. Jan, 2012 | Pubmed ID: 22052706 Spider Silk-based Gene Carriers for Tumor Cell-specific Delivery Bioconjugate Chemistry. Aug, 2011 | Pubmed ID: 21739966 State of Water, Molecular Structure, and Cytotoxicity of Silk Hydrogels Biomacromolecules. Jun, 2011 | Pubmed ID: 21517113 AFM Study of Morphology and Mechanical Properties of a Chimeric Spider Silk and Bone Sialoprotein Protein for Bone Regeneration Biomacromolecules. May, 2011 | Pubmed ID: 21370930 Differences in Cytotoxicity of β-sheet Peptides Originated from Silk and Amyloid β Macromolecular Bioscience. Jan, 2011 | Pubmed ID: 20954203 Quantifying Osteogenic Cell Degradation of Silk Biomaterials Biomacromolecules. Dec, 2010 | Pubmed ID: 21105641 Silk-Based Gene Carriers with Cell Membrane Destabilizing Peptides Biomacromolecules. Oct, 2010 | Pubmed ID: 20942485 Gene Delivery Mediated by Recombinant Silk Proteins Containing Cationic and Cell Binding Motifs Journal of Controlled Release : Official Journal of the Controlled Release Society. Aug, 2010 | Pubmed ID: 20457191 Silk-based Delivery Systems of Bioactive Molecules Advanced Drug Delivery Reviews. Dec, 2010 | Pubmed ID: 20298729 Surface-patterned Electrode Bioreactor for Electrical Stimulation Lab on a Chip. Mar, 2010 | Pubmed ID: 20221556 Mechanisms of Enzymatic Degradation of Amyloid Beta Microfibrils Generating Nanofilaments and Nanospheres Related to Cytotoxicity Biochemistry. Apr, 2010 | Pubmed ID: 20196618 Reinforcing Silk Scaffolds with Silk Particles Macromolecular Bioscience. Jun, 2010 | Pubmed ID: 20166230 Mechanism of Enzymatic Degradation of Beta-sheet Crystals Biomaterials. Apr, 2010 | Pubmed ID: 20044136 Expression, Cross-linking, and Characterization of Recombinant Chitin Binding Resilin Biomacromolecules. Dec, 2009 | Pubmed ID: 19928816 Bioengineered Silk Protein-based Gene Delivery Systems Biomaterials. Oct, 2009 | Pubmed ID: 19577803 Enzymatic Degradation of Monolayer for Poly(lactide) Revealed by Real-time Atomic Force Microscopy: Effects of Stereochemical Structure, Molecular Weight, and Molecular Branches on Hydrolysis Rates Biomacromolecules. Aug, 2008 | Pubmed ID: 18636774 Branched Poly(lactide) Synthesized by Enzymatic Polymerization: Effects of Molecular Branches and Stereochemistry on Enzymatic Degradation and Alkaline Hydrolysis Biomacromolecules. Oct, 2007 | Pubmed ID: 17722879 Adsorption and Hydrolysis Reactions of Poly(hydroxybutyric Acid) Depolymerases Secreted from Ralstonia Pickettii T1 and Penicillium Funiculosum Onto Poly[(R)-3-hydroxybutyric Acid] Biomacromolecules. Jul, 2007 | Pubmed ID: 17547455 Nonspecific Hydrophobic Interactions of a Repressor Protein, PhaR, with Poly[(R)-3-hydroxybutyrate] Film Studied with a Quartz Crystal Microbalance Biomacromolecules. Aug, 2006 | Pubmed ID: 16903695 Adsorption of Biopolyester Depolymerase on Silicon Wafer and Poly[(R)-3-hydroxybutyric Acid] Single Crystal Revealed by Real-time AFM Macromolecular Bioscience. Jan, 2006 | Pubmed ID: 16374769 Enzymatic Degradation Processes of Poly[(R)-3-hydroxybutyric Acid] and Poly[(R)-3-hydroxybutyric Acid-co-(R)-3-hydroxyvaleric Acid] Single Crystals Revealed by Atomic Force Microscopy: Effects of Molecular Weight and Second-monomer Composition on Erosion Rates Biomacromolecules. Jul-Aug, 2005 | Pubmed ID: 16004439 Biosynthesis of Polyhydroxyalkanoate (PHA) Copolymer from Fructose Using Wild-type and Laboratory-evolved PHA Synthases Macromolecular Bioscience. Feb, 2005 | Pubmed ID: 15729719 Enzymatic Degradation Processes of Lamellar Crystals in Thin Films for Poly[(R)-3-hydroxybutyric Acid] and Its Copolymers Revealed by Real-time Atomic Force Microscopy Biomacromolecules. Nov-Dec, 2004 | Pubmed ID: 15530032 In-situ Atomic Force Microscopy Observation of Enzymatic Degradation in Poly(hydroxyalkanoic Acid) Thin Films: Normal and Constrained Conditions Macromolecular Bioscience. Mar, 2004 | Pubmed ID: 15468218 Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants Jo-Ann Chuah1, Yoko Horii1, Keiji Numata1 1Enzyme Research Team, RIKEN Center for Sustainable Resource Science JoVE 54972 Genetics
Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants Jo-Ann Chuah1, Yoko Horii1, Keiji Numata1 1Enzyme Research Team, RIKEN Center for Sustainable Resource Science JoVE 54972 Genetics