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High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM

January 26th, 2024
*1, *1, *1,2, 1, 1,3, 1, 1,4,5, 1,4,6, 1,4,5
1European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, 2Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, 3Department of Physics, University of Pisa, 4National Research Council - National Institute of Optics (CNR-INO), 5Department of Physics and Astronomy, University of Florence, 6Department of Biology, University of Florence
* These authors contributed equally

Summary
Automatic Translation

본 프로토콜은 (SWITCH - H 2O2 - Antigen Retrieval - 2,2'-thiodiethanol [TDE]) SHORT 조직 형질 전환 기법과 광시트 형광 현미경 이미징을 일상적으로 높은 처리량 프로토콜로 결합하여 수십 개의 사후 인간 뇌 절편의 신속하고 동시적인 광학 투명화, 다중 라운드 라벨링 및 3D 체적 재구성을 위한 단계별 절차를 제공합니다.

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